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A Structural Basis for Reversible Photoswitching of Absorbance Spectra in Red Fluorescent Protein rsTagRFP.

Posted from Journal of Molecular Biology 99 days ago
Molecular Biology : Molecular Biology Journals

Publication Date: 2012 Jan 30 PMID: 22310052Authors: Pletnev, S. - Subach, F. V. - Dauter, Z. - Wlodawer, A. - Verkhusha, V. V.Journal: J Mol BiolrsTagRFP the first monomeric red fluorescent protein (FP) with reversibly photoswitchable absorbance spectra. The switching is realized by irradiation of rsTagRFP with blue (440 nm) and yellow (567 nm) light, turning the protein fluorescence ON and OFF, respectively. It is perhaps the most useful probe in this color class that has yet been reported. Because of the photoswitchable absorbance, rsTagRFP can be used as an acceptor in photochromic Forster... (original story)