Posted from Journal of Molecular Biology 99 days ago
Molecular Biology : Molecular Biology Journals
Publication Date: 2012 Jan 30 PMID: 22310045Authors: Qi, H. - Lu, H. - Qiu, H. J. - Petrenko, V. - Liu, A.Journal: J Mol BiolPhagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural and functional proteins necessary to accomplish the life cycle of phagemid are provided by the helper phage. In addition, other elements such as molecular tags and selective markers are introduced into the phagemids to facilitate the subsequent operations, such as gene manipulation and protein puri...
(original story)