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Phagemid Vectors for Phage Display: Properties, Characteristics and Construction.

Publication Date: 2012 Jan 30 PMID: 22310045Authors: Qi, H. - Lu, H. - Qiu, H. J. - Petrenko, V. - Liu, A.Journal: J Mol BiolPhagemids are filamentous-phage-derived vectors containing the replication origin of a plasmid. Phagemids usually encode no or only one kind of coat proteins. Other structural... (original story)

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A Structural Basis for Reversible Photoswitching of Absorbance Spectra in Red Fluorescent Protein rsTagRFP.

Publication Date: 2012 Jan 30 PMID: 22310052Authors: Pletnev, S. - Subach, F. V. - Dauter, Z. - Wlodawer, A. - Verkhusha, V. V.Journal: J Mol BiolrsTagRFP the first monomeric red fluorescent protein (FP) with reversibly photoswitchable absorbance spectra. The switching is realized by irradiation of ... (original story)

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A New Approach for the Joint Analysis of Multiple Chip-Seq Libraries with Application to Histone Modification

Most approaches for analyzing ChIP-Seq data are focused on inferring exact protein binding sites from a single library. However, frequently multiple ChIP-Seq libraries derived from differing cell lines or tissue types from the same individual may be available. In such a situation, a separate analysi... (original story)

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The Effect of Abeta on IAPP Aggregation in the Presence of an Isolated beta-Cell Membrane.

Publication Date: 2012 Feb 1 PMID: 22321797Authors: Seeliger, J. - Weise, K. - Opitz, N. - Winter, R.Journal: J Mol BiolFibrillar aggregates of the islet amyloid polypeptide (IAPP) and amyloid-beta (Abeta) are known to deposit at pancreatic beta-cells and neuronal cells and are associated with the c... (original story)

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CommunicationsNickel Binding and [NiFe]-Hydrogenase Maturation by the Metallochaperone SlyD with a Single Metal-Binding Site in Escherichia coli.

Publication Date: 2012 Jan 30 PMID: 22310044Authors: Kaluarachchi, H. - Altenstein, M. - Sugumar, S. R. - Balbach, J. - Zamble, D. B. - Haupt, C.Journal: J Mol BiolSlyD (sensitive to lysis D) is a nickel metallochaperone involved in the maturation of [NiFe]-hydrogenases in Escherichia coli (E. coli)... (original story)

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Micrococcal Nuclease Does Not Substantially Bias Nucleosome Mapping.

Publication Date: 2012 Jan 30 PMID: 22310051Authors: Allan, J. - Fraser, R. M. - Owen-Hughes, T. - Keszenman-Pereyra, D.Journal: J Mol BiolWe have mapped sequence-directed nucleosome positioning on genomic DNA molecules using high-throughput sequencing. Chromatins, prepared by reconstitution with ei... (original story)

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Hypermutation of apoB mRNA by rat APOBEC-1 over-expression mimicks APOBEC-3 hypermutation.

Publication Date: 2012 Feb 9 PMID: 22326345Authors: Chen, Z. - Eggerman, T. L. - Bocharov, A. V. - Baranova, I. N. - Vishnyakova, T. G. - Kurlander, R. J. - Csako, G. - Patterson, A. P.Journal: J Mol BiolAPOBEC-3 proteins induce C-to-U hypermutations in the viral genome of various viruses and have b... (original story)

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Multiple Roles of Heparin in the Aggregation of p25alpha

Publication Date: 2012 Feb 3 PMID: 22326478Authors: Nielsen, S. B. - Yde, P. - Giehm, L. - Sundbye, S. - Matthiesen, J. - Jensen, M. H. - Jensen, P. H. - Otzen, D. E.Journal: J Mol BiolThe 219-residue protein p25alpha stimulates the fibrillation of alpha-synuclein (alphaSN) in vitro and colocalizes ... (original story)

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Graph Selection with GGMselect

Applications on inference of biological networks have raised a strong interest in the problem of graph estimation in high-dimensional Gaussian graphical models. To handle this problem, we propose a two-stage procedure which first builds a family of candidate graphs from the data, and then selects on... (original story)

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Driving forces and structural determinants of steric zipper peptide oligomer formation elucidated by atomistic simulations.

Publication Date: 2012 Feb 8 PMID: 22326493Authors: Matthes, D. - Gapsys, V. - de Groot, B. L.Journal: J Mol BiolUnderstanding the structural and energetic requirements of non-fibrillar oligomer formation harbors the potential to decipher an important, yet still elusive part of amyloidogenic peptide... (original story)

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